Resource Type:
Project
Name:
Hybrid Poplar H11-11 Systemic Herbivory Response Dataset 2
Short Description:
We investigated the transcriptional response of hybrid poplar (Populus trichocarpa x deltoides) source and sink leaves to simulated herbivory (mechanical wounding plus the application of Malacosoma disstria oral secretions) over a 24 hour time course. Experiments were conducted using clonal trees under greenhouse conditions at the University of British Columbia. We used the 15.5K poplar cDNA microarray platform previously described by Ralph et al. (Molecular Ecology 2006, 15:1275-1297). Differentially expressed genes were determined using three criteria: fold-change between treated and untreated control leaves > 1.5-fold, P value 3,000 differentially expressed genes in response to simulated insect feeding damage, which possess distinct source/sink and treated/systemic patterns. Overall design: For simulated herbivory analysis, we compared RNA from systemic sink leaves of four individual treated trees versus four individual control trees, with dye balance. We also compared pooled RNA from systemic sink leaves of five treated or five untreated trees, for a total of 8 slides. Based on an earlier study using pooled biological replicates (90 trees) to examine a large combination of leaf types and time points, we selected the peak response group for the simulated herbivory treatment for further investigation using individual tree replicates (systemic sink leaves, 2hrs).
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Database Cross Reference:
GenBank GI:122855
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