# Katharina J. Hoff # November 25th 2020 # Species specific AUGUSTUS parameters for Dunaliella salina Augustus parameter set name: Dunaliella_salina Lineage: Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; Chlamydomonadales; Dunaliellaceae; Dunaliella This parameter set was created with BRAKER on the basis of genome assembly https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/284/615/GCA_002284615.2_Dunsal1_v._2/GCA_002284615.2_Dunsal1_v._2_genomic.fna.gz and OrthoDb proteins https://v100.orthodb.org/download/odb10_plants_fasta.tar.gz Call: braker.pl --genome=genome.fa --prot_seq=proteins.fasta --softmasking --cores=8 --species=Dunaliella_salina --epmode Flanking region size: 5564 Number of generated training genes: 3428 nonredundant genes, 300 set aside for testing, 300 set aside for optimize_augustus.pl time consuming steps Initial accuracy on test set: 0.688666666666667 Accuracy on test set after optimize_augustus.pl: 0.690333333333333 With this parameter set, BRAKER predicted 16461 genes (Augustus with hints in the target genome). For comparison, the GenBank annotation contains 18740 genes. This parameter set was generated in a fully automated fashion for supporing the POMPU project (http://www.pompu-project.de/). If using this parameter set, please cite: Bruna, T., Hoff, K., Stanke, M., Lomsadze, A., & Borodovsky, M. (2020). BRAKER2: Automatic Eukaryotic Genome Annotation with GeneMark-EP+ and AUGUSTUS Supported by a Protein Database. bioRxiv. https://www.biorxiv.org/content/10.1101/2020.08.10.245134v1.abstract