# Katharina J. Hoff # November 25th 2020 # Species specific AUGUSTUS parameters for Monoraphidium neglectum Augustus parameter set name: Monoraphidium_neglectum Lineage: Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; Sphaeropleales; Selenastraceae; Monoraphidium This parameter set was created with BRAKER on the basis of genome assembly https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/611/645/GCF_000611645.1_mono_v1/GCF_000611645.1_mono_v1_genomic.fna.gz and OrthoDb proteins https://v100.orthodb.org/download/odb10_plants_fasta.tar.gz Call: braker.pl --genome=genome.fa --prot_seq=proteins.fasta --softmasking --cores=8 --species=Monoraphidium neglectum --epmode Flanking region size: 1420 Number of generated training genes: 2913 nonredundant genes, 300 set aside for testing, 300 set aside for optimize_augustus.pl time consuming steps Initial accuracy on test set: 0.855 Accuracy on test set after optimize_augustus.pl: 0.871733333333333 With this parameter set, BRAKER predicted 19514 genes (Augustus with hints in the target genome). For comparison, the RefSeq annotation contains 16755 genes. This parameter set was generated in a fully automated fashion for supporing the POMPU project (http://www.pompu-project.de/). If using this parameter set, please cite: Bruna, T., Hoff, K., Stanke, M., Lomsadze, A., & Borodovsky, M. (2020). BRAKER2: Automatic Eukaryotic Genome Annotation with GeneMark-EP+ and AUGUSTUS Supported by a Protein Database. bioRxiv. https://www.biorxiv.org/content/10.1101/2020.08.10.245134v1.abstract