# Katharina J. Hoff # November 28th 2020 # Species specific AUGUSTUS parameters for Rhopilema esculentum Augustus parameter set name: Rhopilema_esculentum Lineage: Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Cnidaria; Scyphozoa; Rhizostomeae; Rhizostomatidae; Rhopilema This parameter set was created with BRAKER on the basis of genome assembly https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/013/076/305/GCA_013076305.1_ASM1307630v1/GCA_013076305.1_ASM1307630v1_genomic.fna.gz and OrthoDb proteins https://v100.orthodb.org/download/odb10_metazoa_fasta.tar.gz Call: braker.pl --genome=genome.fa --prot_seq=proteins.fasta --softmasking --cores=8 --species=Rhopilema_esculentum --epmode Flanking region size: 2614 Number of generated training genes: 2722 nonredundant genes, 300 set aside for testing, 300 set aside for optimize_augustus.pl time consuming steps Initial accuracy on test set: 0.820133333333333 Accuracy on test set after optimize_augustus.pl: 0.8302 With this parameter set, BRAKER predicted 24150 genes (Augustus with hints in the target genome). To our knowledge, this might be the first gene set for this genome assembly. The gene set is available upon request from katharina.hoff@uni-greifswald.de. This parameter set was generated in a fully automated fashion for supporing the POMPU project (http://www.pompu-project.de/). If using this parameter set, please cite: Bruna, T., Hoff, K., Stanke, M., Lomsadze, A., & Borodovsky, M. (2020). BRAKER2: Automatic Eukaryotic Genome Annotation with GeneMark-EP+ and AUGUSTUS Supported by a Protein Database. bioRxiv. https://www.biorxiv.org/content/10.1101/2020.08.10.245134v1.abstract